IBA : Protein Tools

Features and benefits
Strep-Well HT Purification Plates
Compatibility with robotic systems
Procedure
Strep-Well HT Purification Starter Kits
Order information
Custom service
Purification Protocol for Strep-Well HT purification plates (pdf, 239 KB)

The perfect solution for automated, high-throughput purification of Strep-tag^® proteins

Features and benefits

Simultaneous purification of up to 96 different Strep-tag proteins
Up to 200 µg highly pure Strep-tag protein per well
Convenient ready-to-use format
Pre-filled with immobilized Strep-Tactin^®
Time-saving
Compatible with vacuum manifolds and robotic systems

96 protein samples purified in parallel

Two different Strep-Well HT Purification plates are available: Strep-Well HT 25 Purification Plates provide automated protein purification of up to 100 µg recombinant protein per well, while Strep-Well HT 50 Purification Plates provide purification of up to 200 µg recombinant protein per well of 96 samples in parallel.
These 96-well affinity chromatography modules are the perfect solution for rapid or high-throughput purification of Strep-tag proteins. The plates are pre-loaded with Strep-Tactin and simply have to be re-hydrated and equilibrated before usage.

Strep-tag vector constructs for the expression of GAPDH from S. aureus (37.5 kDa), GFP from A. victoria (28.1 kDa), human GSHH (23.5 kDa) and Azurin from P. aeruginosa (15.1 kDa) were transformed into E. coli and plated on selective medium. 96 colonies were randomly picked to inoculate 5 ml cultures. Protein expression was induced by addition of anhydrotetracycline or IPTG. Cells were harvested, lysed and Strep-tag proteins were purified on a Strep-Tactin HT Purification Plate. 8 µl of 20 elution fractions were loaded on a SDS-PAGE, gel was stained with Coomassie.

Compatibility with robotic systems

The Strep-Well HT Purification Plate is compatible with robotic sample processing systems, such as the Genesis RSP/RWS Separation System (Tecan), the MultiPROBE II (Packard BioScience), the BioRobot workstations (Qiagen) and the Biomek 2000/FX robots (Beckman Coulter), but can also be used with standard vacuum manifolds for manual sample processing.


Genesis RSP/RWS Separation System (Photo kindly provided by Tecan).	BioRobot workstation (Photo kindly provided by Qiagen).	Biomek FX robot (Photo kindly provided by Beckman Coulter).

Procedure

The procedure uses a 96-well filter plate to clear bacterial lysates by vacuum filtration.

The cultures are chemically lysed.
The lysates are cleared by vacuum filtration through the 96-well filter plate.
The cleared protein lysates are applied to the Strep-Well HT Purification Plate.
After washing, the purified proteins are eluted in 300-450 µl elution buffer.

To prevent cross-contamination of the samples a 96-well wash plate is available.
The eluates are collected in a 96-well receiver plate.
Washing and elution steps are carried out on a vacuum manifold. Ready-to-run protocols are provided.

Strep-Well HT Purification Starter Kits

For a first evaluation we are offering a Strep-Well HT Purification Starter Kit in two versions, one with a Strep-Well HT 25 Purification Plate allowing purification of up to 100 µg protein per well and one with a Strep-Well HT 50 Purification Plate for the purification of up to 200 µg protein per well. Also included in the kits are one 96-well filter, wash and receiver plate each as well as the required buffers for processing 96 samples.
Larger amounts of plates and buffers are available separately (see below).

Strep-Well HT Purification Starter Kit

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Order information
see also "how to order"

product	amount	cat. no.	order & prices
Strep-Well HT 25 Purification Starter Kit	1 kit	2-1700-000
Strep-Well HT 50 Purification Starter Kit	1 kit	2-1701-000
Strep-Well HT 25 Purification Plates	1 plate	2-1725-001
Strep-Well HT 25 Purification Plates	10 plates	2-1725-010
Strep-Well HT 25 Purification Plates	25 plates	2-1725-025
Strep-Well HT 25 Purification Plates	100 plates	2-1725-100
Strep-Well HT 50 Purification Plates	1 plate	2-1750-001
Strep-Well HT 50 Purification Plates	10 plates	2-1750-011
Strep-Well HT 50 Purification Plates	25 plates	2-1750-025
Strep-Well HT 50 Purification Plates	100 plates	2-1750-100
Strep-Well HT buffer set for 10 plates		2-1702-000
Strep-Well HT filter plates	10 plates	2-1705-010
Strep-Well HT wash plates	10 plates	2-1706-010
Strep-Well HT receiver plates	10 plates	2-1707-010

Further Strep-Well HT Purification Plate formats on request.

Strep-Well HT Purification System

Features and benefits

96 protein samples purified in parallel

Compatibility with robotic systems

Procedure

Strep-Well HT Purification Starter Kits