Strep-tag® II antibodies

StrepMAB-Classic1

StrepMAB-Classic, the monoclonal antibody against Strep-tag II, is recommended for the following applications:

  • Immune fluorescence
  • FACS
  • Detection in ELISA

It is available purified and lyophilized (2-1507-001). Alternatively, we offer cell culture supernatant (2-1508-025 and 2-1508-050).


Specifications of StrepMAB-Classic, 2-1507-001

Purification purified by affinity chromatography
Host animal/Subclass Mouse/IgG1
Specificity N- or C-terminal or internal Strep-tag II
Form lyophilized from NH4HCO3 with 10 mg sucrose per mg antibody
Stability 18 months after shipment
Storage stored at -20 °C
Shipment RT

1Formerly: Strep-tag II specific monoclonal antibody
For more details and order info please see below




Secondary antibody for detection of StrepMAB-Classic:
Rabbit anti Mouse pAb, horseradish peroxidase (HRP) conjugated

Rabbit anti Mouse pAb, HRP conjugated, is recommended for detection of

  • bound mouse monoclonal antibodies, like StrepMAB-Classic, in Western blots (chromogenic reaction),
  • mouse monoclonal antibodies, like StrepMAB-Classic, in microplate assays

Specifications of Rabbit anti Mouse pAb, HRP conjugated, 2-1591-001

Description Purified polyclonal rabbit anti mouse immunoglobulin fraction conjugated to horseradish peroxidase (HRP).
Specifity The antibody used for conjugation has not been absorbed to remove cross-reactions to immunoglobulin from other species. Therefore the conjugate shows reactions with mouse immunoglobulin of all classes
Form Dissolved in 50 mM Tris/HCl 15 mM NaN3, pH 7.2
Concentration of conjugate 1.3 mg/ml
Amount 0.5 ml
Dilution
  • Dilute 1:1000 for chromogenic detection of bound mouse monoclonal antibodies in Western Blots
  • Dilute 1:2000 (or titrate up to 1:10,000) for detection of mouse monoclonal antibodies in microplate assays
Storage 2 - 8 °C
Stability 6 months after shipping
Shipment RT

For more details and order info please see below





StrepMAB-Classic horseradish peroxidase (HRP) conjugate

The StrepMAB-Classic HRP conjugate is recommended for detection of Strep-tag II proteins in Western blots without the need for secondary antibodies. The conjugate is useful for both chemiluminescence and chromogenic detection.


Specifications of StrepMAB-Classic horseradish peroxidase
(HRP) conjugate, 2-1509-001

Purification purified by affinity chromatography
HRP conjugate conjugated to 3-3.5 mols horseradish peroxidase per mol IgG
Host animal/Subclass Mouse/IgG1
Specificity N- or C-terminal or internal Strep-tag II
Form suspension in PBS with 0.5% BSA and 0.01 % thimerosal
Stability 18 months after shipment
Storage stored at 4 °C, do not freeze!
Shipment RT

For more details and order info please see below


Need buying or technical advice?
Contact a protein expert!
  • +49(0)551 50672-0
  • Online
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Order information
see also "how to order"

product amount cat. no. order & prices
StrepMAB-Classic1
purified, lyophilized 100 µg for 50 Western blots2 2-1507-001 online order
crude cell culture supernatant for 25 Western blots2 2-1508-025 online order
crude cell culture supernatant for 50 Western blots2 2-1508-050 online order
HRP conjugate 75 µl for
25-30 Western blots2		 2-1509-001 online order
Rabbit anti Mouse pAb, HRP conjugated
purified, dissolved 0.5 ml 2-1591-001 online order


1Formerly: Strep-tag II specific monoclonal antibody
2Western blot: 10 ml incubation volume each
Please note, that StrepMAB-Classic is not recommended for capturing of Strep-tagged proteins on solid phases. Use StrepMAB-Immo for protein capturing.




Especially suited for protein interaction analysis on Biacore1 systems!

StrepMAB-Immo

High-affinity Strep-tag® II specific monoclonal antibody for capturing Strep-tag fusion proteins on solid phases

StrepMAB-Immo is a murine, high-affinity Strep-tag II specific monoclonal antibody which is especially suited for stable, mild and oriented capturing of Strep-tag II fusion proteins on solid phases. To realize this, the antibody can be immobilized on e.g. microplates, Biacore CM5 sensor chips or other biochips.
The nearly irreversible binding is achieved both for fusion proteins carrying a C- or
N- terminal Strep-tag. The Strep-tag must be N-terminally extended by a SerAla linker (recombinant protein - SA-WSHPQFEK
or SA-WSHPQFEK - recombinant protein). Please refer also to IBA's expression vectors.



Specifications of StrepMAB-Immo

Host animal/Subclass Mouse/IgG1
Specifity Nearly irreversible binding of C- or N-terminally fused Strep-tag II, which is N-terminally extended by "SerAla" ** like recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein. Proteins containing Strep-tag II with
N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far.
Form
binding capacity		 lyophilized from NH4HCO3 with 10 mg sucrose per mg antibody
(determined for bacterial alkaline phosphatase)
Stability 24 months after shipment
Storage -20 °C
Shipment RT
**pASK-/pEXPR- and pPR-IBA vectors with N-term One-/Strep-tag II do NOT have "SerAla"-Linker but the
StarGate vectors (carrying Strep-tag II and One-Strep-tag) are equipped with the SA-Linker.

During the washing phase, the recombinant Strep-tag protein of interest remains tightly bound to StrepMAB-Immo, while a significant amount of Strep-tag protein is washed off using the competitive antibody.

Application protocol (pdf; 243 KB)



Need buying or technical advice?
Contact a protein expert!
  • +49(0)551 50672-0
  • Online
  • E-mail (please indicate your complete address)


Order information
see also "how to order"

product amount cat. no. order & prices
StrepMAB-Immo 100 µg 2-1517-001 online order



Please note, that the antibody is not recommended for detection of Strep-tag II fusion proteins in Western blots. Use StrepMAB-Classic HRP conjugate for Western blots.


1) For more information on Biacore applications refer also to www.biacore.de.


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