IBA : Protein Tools

Get started with Strep-tag

Attractive offers for newcomers are our Strep-tag Starter Kits containing all essential reagents required for expression in E. coli, purification and detection of Strep-tag proteins. While the regular Starter Kit contains one Strep-Tactin purification column, the Starter Kit 3C includes 3 different columns with Strep-Tactin^®immobilized to Sepharose^®, MacroPrep^® and Superflow^®, respectively, allowing the evaluation of the optimal resin for your particular protein of interest. See also Strep-Tactin Column Evaluation Set.

We offer two different Strep-tag Starter Kits "Cartridge H-PR" for protein purification under low pressure allowing fast and convenient purification of your Strep-tag protein: the Strep-tag Starter Kit "Cartridge H-PR" Superflow is equipped with a Strep-Tactin Superflow cartridge H-PR (1 ml) with 10-32 connection, the Strep-tag Starter Kit "Cartridge H-PR" MacroPrep is equipped with a Strep-Tactin MacroPrep cartridge H-PR (1ml) with 10-32 connection. Both cartridges can be directly connected to workstations with a 10-32 connection (HPLC and Äkta), adapters for connection to other devices are also available.

The kits include the complete set of reagents essential for the expression, purification and detection of Strep-tag proteins.

For the appropriate expression vectors please click here

See also applications and specifications of the columns.

For your convenience: Primer D'Signer software


Strep-tag Starter Kit with one purification column	Strep-tag Starter Kit 3C with 3 different purification columns	Strep-tag Starter Kits "Cartridge H-PR" with one cartridge for purification under low pressure. We offer two kits: The Strep-tag Starter Kit "Cartridge H-PR" Superflow is equipped with a Strep-Tactin Superflow cartridge H-PR (1 ml) with 10-32 connection, the Strep-tag Starter Kit "Cartridge H-PR" Macroprep is equipped with a Strep-Tactin Macroprep cartridge H-PR (1ml) with 10-32 connection. Adapters for both types of cartridges are also available.

Protocols for download

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Strep-tag Starter Kit 1 purification column	Strep-tag Starter Kit 3C 3 different purification columns	Strep-tag Starter Kit "Cartridge H-PR" Superflow 1 Strep-Tactin Superflow H-PR cartridge for highly pressure-resistant (H-PR) purification (up to 20 bar), use of flow restrictor possible	Strep-tag Starter Kit "Cartridge" Macroprep 1 Strep-Tactin Macroprep cartridge for purification under low pressure
Kit contents:	Kit contents:	Kit contents:	Kit contents:
1 ready-to-use column with Strep-Tactin Sepharose, 1 ml	3 ready-to-use columns with Strep-Tactin Sepharose, Strep-Tactin MacroPrep and Strep-Tactin Superflow, respectively (1ml each)	1 Strep-Tactin Superflow H-PR cartridge (1 ml); optionally 4 adapter sets for connection to Luer-lock syringes and various chromatography systems and workstations	1 Strep-Tactin MacroPrep cartridge, 1 ml; optionally 4 adapter sets for connection to peristaltic pump chromatography systems or FPLC/HPLC workstations
Included in both kits (sufficient for 8 applications): control plasmid with 15 kD protein insert anhydrotetracycline for induction of expression fractionation buffer for the preparation of a periplasmic extract washing buffer for column chromatography or for the preparation of a cytoplasmic extract elution buffer for displacing the Strep-tag protein from the column column regeneration buffer (with HABA) Strep-Tactin horse radish peroxidase (HRP) conjugate for Western blot detection Comprehensive manual
Order no. 2-1101-000	Order no. 2-1102-001	2-1115-000 Strep-tag Starter Kit "Cartridge H-PR" Superflow with adapters	2-1113-000 Strep-tag Starter Kit "Cartridge H-PR" MacroPrep with adapters
order & prices	order & prices	order & prices	order & prices
		2-1116-000 Strep-tag Starter Kit "Cartridge H-PR" Superflow without adapters	2-1114-000 Strep-tag Starter Kit "Cartridge H-PR" MacroPrep without adapters
		order & prices	order & prices

Strep-tag® Starter Kits

Get started with Strep-tag

Strep-tag^® Starter Kits